OptoPlate-96 Bugaj Lab

The OptoPlate-96 permits high-throughput three-color optogenetics experiments with simultaneous manipulation of red- and blue-light-sensitive optogenetic probes. Its design prevents cross-illumination between microwells in 96-well plates. An active cooling system minimizes sample heating during light-intensive experiments. Each well position can also illuminate 4-wells of a 384-well plate.

EXPERIMENTAL USE

Multi-colour optogenetics provides a method for analysing multi-pathway cell networks in a systematic manner.

• Immunofluorescence analysis
• Fits both 96-well plates and 384-well plates
  
• Three colours to stimulate common mammalian cell optogenetics probes

KEY FEATURES

• Offers data-rich experiments with precise perturbations and high-throughput
  
• Three wavelengths: Far-Red (780nm), Red (630 nm) and Blue (470 nm)
  
• Independent control of stimulation colours, intensity, dynamics, and time points
• Arduino microcontroller stores and executes user-defined illumination profiles

CONTENTS OF THE SET

• Light isolated lid
• 96 or 384 well plate
• OptoPlate illuminator: 470, 630, and 780 nm (standard: others on request)
  
• Thermal pad
• Aluminium plate
• Heat sink with fan
  
• Base plate
• Power supplies (US only)

PUBLICATION

LJ Bugaj & WA Lim (2019). High-throughput multicolor optogenetics in microwell plates. Nature Protocols, 14, 2205–2228.

Thomas, OS, Hörner, M & Weber, W (2020). A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nature Protocols. 

Documentation  Source Code